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KMID : 1007520130220061683
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Food Science and Biotechnology
2013 Volume.22 No. 6 p.1683 ~ p.1690
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Pomelo Citrus grandis (L.) Osbeck Peel as an Economical Alternative Substrate for Fungal Pectinase Production
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I. Darah
M. M. J. Taufiq
S. H. Lim
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Abstract
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Polygalacturonase production by Aspergillus niger LFP-1 was studied in solid state fermentation (SSF) using pomelo (Citrus grandis) peel as a substrate. This local agricultural waste product is rich in lignocellulolytic material, including pectin, which can act as an inducer of pectinase production. Using the parameters of 5 g of 0.75 mm (particle size) pomelo peel as a substrate, moisture content ratio of 1:1 (w/v), inoculum size of 1×107 spores/ mL, cultivation temperature of 30ºC, and no mixing, static fermentation conditions with addition of 1.2% ammonium nitrate produced the highest polygalacturonase production rate of 8.90 U/g of substrate and a fungal growth rate of 2.07mg of glucosamine/g of substrate on day 5 of cultivation. A large increase (1,434.5%) in enzyme production occurred after improvement of the growth parameters. Under optimum bioprocess conditions, pomelo peel can be used as solid substrate for production of pectinase.
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KEYWORD
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pomelo peel, pectinase, solid state fermentation, optimization, Aspergillus niger
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